A Molecular Mechanism of Bacterial Flagellar Motor Switching
نویسندگان
چکیده
منابع مشابه
A molecular mechanism of bacterial flagellar motor switching.
The high-resolution structures of nearly all the proteins that comprise the bacterial flagellar motor switch complex have been solved; yet a clear picture of the switching mechanism has not emerged. Here, we used NMR to characterize the interaction modes and solution properties of a number of these proteins, including several soluble fragments of the flagellar motor proteins FliM and FliG, and ...
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The direction of flagellar rotation is regulated by a rotor-mounted protein assembly, termed the "switch complex," formed from multiple copies of the proteins FliG, FliM, and FliN. The structures of major parts of these proteins are known, and the overall organization of proteins in the complex has been elucidated previously using a combination of protein-binding, mutational, and cross-linking ...
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The bacterial flagellar motor is a large rotary molecular machine that propels swimming bacteria, powered by a transmembrane electrochemical potential difference. It consists of an ∼50-nm rotor and up to ∼10 independent stators anchored to the cell wall. We measured torque-speed relationships of single-stator motors under 25 different combinations of electrical and chemical potential. All 25 to...
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Switching dynamics of flagellar motors of Escherichia coli is commonly observed through markers attached to the flagellar filaments. To eliminate possible complications resulting from the conformational transitions of these filaments and to look at the output of motors more directly, we monitored motor rotation by attaching nanogold spheres to the hooks of cells lacking filaments. We observed e...
متن کاملThe switching dynamics of the bacterial flagellar motor - Supporting Information
The model for the stator-rotor interaction is discussed in the sections The stator-rotor interaction and The rotor switching dynamics of the main text. The model is based on the model of Oster and Blair and coworkers [1, 2], but extended to include the conformational transitions of the rotor protein complex. Here, we discuss aspects of the model that are not discussed in the main text. But, for...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2009
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2009.02.004